| 摘要： |
Background: Arachidonic acid (ARA), which is omega-6 polyunsaturated fatty acid, has a wide range of biological activities and is an essential component of cellular membranes in some human tissues. Mortierella alpina is the best strain for industrial production of ARA. To increase its yield of arachidonic acid, heavy ion beam irradiation mutagenesis of Mortierella alpina was carried out in combination with triclosan and octyl gallate treatment. Results: The obtained mutant strain F-23 ultimately achieved an ARA yield of 5.26 g L-1, which is 3.24 times higher than that of the wild-type strain. In addition, quantitative real-time PCR confirmed that the expression levels of fatty acid synthase (FAS), Delta 5-desaturase, Delta 6-desaturase, and Delta 9-desaturase were all significantly up-regulated in the mutant F-23 strain, especially Delta 6- and Delta 9-desaturase, which were up-regulated 3- and 2-fold, respectively. Conclusions: This study confirmed a feasible mutagenesis breeding strategy for improving ARA production and provided a mutant of Mortierella alpina with high ARA yield. |
