| 第一作者： | Xu, T (Xu, Teng); Han, X (Han, Xiao); Zhu, PF (Zhu, Pengfei); Dai, J (Dai, Jing); Liu, M (Liu, Min); Liu, Y (Liu, Yang); Xu, J (Xu, Jian); Ma, B (Ma, Bo); |
|---|---|
| 联系作者： | Xu, T (Xu, Teng); Han, X (Han, Xiao); Zhu, PF (Zhu, Pengfei); Dai, J (Dai, Jing); Liu, M (Liu, Min); Liu, Y (Liu, Yang); Xu, J (Xu, Jian); Ma, B (Ma, Bo); |
| 发表年度： | 2021 |
| 卷： | 343 |
| 页： | - |
| 摘要： | The available pathogenic bacteria detection and antimicrobial susceptibility testing (AST) are still time-consuming and tedious because of the lengthy culture procedures. Various kinds of systems have promised a great reduction in AST time, however the high-efficiency and user-friendly bacteria enrichment is always a challenge to tackle. Here we introduce a centrifugation-based microfluidic concentrator for milliliter to picoliter bacteria suspension enrichment to achieve a fast and real-time pathogen detection and AST. The concentrator consists of a milliliter-level loading chamber and a picoliter-level enrichment channel. By centrifuging, all pathogen cells in chamber can be condensed into enrichment channel, achieving a milliliter to picoliter enrichment. Thus, the pathogen cells can be detected directly by microscopic observation. More importantly, the AST can be started from original sample without traditional pre-culture steps for the high enrichment efficiency. Since all the cells were confined at the picoliter-level channel tip, detectable growth of phenotype can be easily observed in a few hours, and the different inhibition mechanism on cells from different antibiotics can be clearly observed. The proposed concentrator shows a detection limit of 10(2) cell/mL with 0.5 h of enrichment for detection and another 3 h of micro-culture for AST. |
| 刊物名称： | SENSORS AND ACTUATORS B-CHEMICAL |
| 全文链接： | https://www.sciencedirect.com/science/article/pii/S0925400521006869?via%3Dihub |
