| 摘要： |
In this study, oxygen tolerance of five photohydrogen-producing green algal species, Chlamydomonas moewusii, Chlorella fusca, Chlorella pyrenoidosa, Chlamydomonas reinhardtii and Scenedesmus obliquus, were first evaluated comparatively. These green algae varied in O-2-tolerance. C. pyrenoidosa exhibited notable O-2-tolerance and was investigated comparatively to that of C. reinhardtii. We used an improved O-2-tolerance detection method to eliminate the effects of O-2 dissolving rate. Results showed that the O2I50 values of C. pyrenoidosa cells (in vivo) and cell lysate (in vitro) were 139% and 61% higher than those of C. reinhardtii, respectively. Furthermore, owing to the higher O-2-tolerance, C. pyrenoidosa cells could evolve hydrogen at a higher threshold of O-2 content in the photobiological hydrogen production process and kept releasing H-2 for more than 260 h under continuous illumination compared to 60 h in C. reinhardtii did under the same conditions. (C) 2016 Elsevier B.V. All rights reserved. |
