| 第一作者： | Li, CY (Li, Chunyu); Gong, YH (Gong, Yanhai); Wang, XX (Wang, Xixian); Xu, J (Xu, Jian); Ma, B (Ma, Bo); |
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| 联系作者： | Li, CY (Li, Chunyu); Gong, YH (Gong, Yanhai); Wang, XX (Wang, Xixian); Xu, J (Xu, Jian); Ma, B (Ma, Bo); |
| 发表年度： | 2021 |
| 页： | - |
| 摘要： | An addressable dynamic droplet array (aDDA) is presented that combines the advantages of static droplet arrays and continuous-flow droplet platforms. Modular fabrication is employed to create a self-contained integrated aDDA. All the sample preparation steps, including single-cell isolation, cell lysis, amplification, and product retrieval, are performed in sequence within a sub-nanoliter (approximate to 300 pL) droplet. Sequencing-based validation suggests that aDDA reduces the amplification bias of multiple displacement amplification (MDA) and elevates the percentage of one-yeast-cell genome recovery to 91%, as compared to the average of 26% using conventional, 20 mu L volume MDA reactions. Thus, aDDA is a valuable addition to the toolbox for high-genome-coverage sequencing of single microbial cells. |
| 刊物名称： | SMALL |
| 影响因子： | 10.856 |
| 全文链接： | https://onlinelibrary.wiley.com/doi/10.1002/smll.202100325 |
