| 第一作者： | Su, KL (Su, Kunlong); Wu, ZY (Wu, Zhenying); Liu, YC (Liu, Yuchen); Jiang, SS (Jiang, Shanshan); Ma, DM (Ma, Dongmei); Wang, Y (Wang, Yan); Fu, CX (Fu, Chunxiang); |
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| 联系作者： | Su, KL (Su, Kunlong); Wu, ZY (Wu, Zhenying); Liu, YC (Liu, Yuchen); Jiang, SS (Jiang, Shanshan); Ma, DM (Ma, Dongmei); Wang, Y (Wang, Yan); Fu, CX (Fu, Chunxiang); |
| 发表年度： | 2021 |
| 期： | 9 |
| 卷： | 44 |
| 页： | 3173-3183 |
| 摘要： | Dinitrotoluene (DNT) has been extensively used in manufacturing munitions, polyurethane foams and other important chemical products. However, it is highly toxic and mutagenic to most organisms. Here, we synthesized a codon-optimized bacterial nitroreductase gene, NfsI, for plant expression. The kinetic analysis indicates that the recombinant NfsI can detoxify both 2,4-DNT and its sulfonate (DNTS), while it has a 97.6-fold higher catalytic efficiency for 2,4-DNT than DNTS. Furthermore, we overexpressed NfsI in switchgrass (Panicum virgatum L.), which is a multiple-purpose crop used for fodder and biofuel production as well as phytoremediation. The 2,4-DNT treatment inhibited root elongation of wild-type switchgrass plants and promoted reactive oxygen species (ROS) accumulation in roots. In contrast, overexpression of NfsI in switchgrass significantly alleviated 2,4-DNT-induced root growth inhibition and ROS overproduction. Thus, the NfsI overexpressing transgenic switchgrass plant removed 94.1% 2,4-DNT after 6 days, whose efficiency was 1.7-fold higher than control plants. Moreover, the comparative transcriptome analysis suggests that 22.9% of differentially expressed genes induced by 2,4-DNT may participate in NfsImediated 2,4-DNT detoxification in switchgrass. Our work sheds light on the function of NfsI during DNT phytoremediation for the first time, revealing the application potential of switchgrass plants engineered with NfsI. |
| 刊物名称： | PLANT CELL AND ENVIRONMENT |
| 影响因子： | 5.624 |
| 全文链接： | https://onlinelibrary.wiley.com/doi/10.1111/pce.14099 |
