| 第一作者： | Bhaskar, Y (Bhaskar, Yogendra); Su, XQ (Su, Xiaoquan); Xu, CG (Xu, Chenggang); Xu, JA (Xu, Jian); |
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| 联系作者： | Bhaskar, Y (Bhaskar, Yogendra); Su, XQ (Su, Xiaoquan); Xu, CG (Xu, Chenggang); Xu, JA (Xu, Jian); |
| 发表年度： | 2021 |
| 卷： | 12 |
| 页： | - |
| 摘要： | In selective RNA processing and stabilization (SRPS) operons, stem-loops (SLs) located at the 3 '-UTR region of selected genes can control the stability of the corresponding transcripts and determine the stoichiometry of the operon. Here, for such operons, we developed a computational approach named SLOFE (stem-loop free energy) that identifies the SRPS operons and predicts their transcript- and protein-level stoichiometry at the whole-genome scale using only the genome sequence via the minimum free energy (Delta G) of specific SLs in the intergenic regions within operons. As validated by the experimental approach of differential RNA-Seq, SLOFE identifies genome-wide SRPS operons in Clostridium cellulolyticum with 80% accuracy and reveals that the SRPS mechanism contributes to diverse cellular activities. Moreover, in the identified SRPS operons, SLOFE predicts the transcript- and protein-level stoichiometry, including those encoding cellulosome complexes, ATP synthases, ABC transporter family proteins, and ribosomal proteins. Its accuracy exceeds those of existing in silico approaches in C. cellulolyticum, Clostridium acetobutylicum, Clostridium thermocellum, and Bacillus subtilis. The ability to identify genome-wide SRPS operons and predict their stoichiometry via DNA sequence in silico should facilitate studying the function and evolution of SRPS operons in bacteria. |
| 刊物名称： | FRONTIERS IN MICROBIOLOGY |
| 影响因子： | 4.259 |
| 全文链接： | https://www.frontiersin.org/articles/10.3389/fmicb.2021.673349/full |
