| 第一作者： | Qi, K (Qi, Kuan); Chen, C (Chen, Chao); Yan, F (Yan, Fei); Feng, YG (Feng, Yingang); Bayer, EA (Bayer, Edward A.); Kosugi, A (Kosugi, Akihiko); Cui, Q (Cui, Qiu); Liu, YJ (Liu, Ya-Jun); |
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| 联系作者： | Qi, K (Qi, Kuan); Chen, C (Chen, Chao); Yan, F (Yan, Fei); Feng, YG (Feng, Yingang); Bayer, EA (Bayer, Edward A.); Kosugi, A (Kosugi, Akihiko); Cui, Q (Cui, Qiu); Liu, YJ (Liu, Ya-Jun); |
| 发表年度： | 2021 |
| 卷： | 337 |
| 页： | - |
| 摘要： | Consolidated bio-saccharification (CBS) technology employs cellulosome-producing bacterial cells, rather than fungal cellulases, as biocatalysts for cost-effective production of lignocellulosic sugars. Extracellular beta-glucosidase (BGL) expression in the whole-cell arsenal is indispensable, due to severe cellobiose inhibition of the cellulosome. However, high-level BGL expression in Clostridium thermocellum is challenging, and the optimal BGL production level for efficient cellulose saccharification is currently unknown. Herein, we obtained new CBS biocatalysts by transforming BGL-expressing plasmids into C. thermocellum, which produced abundant BGL proteins and hydrolyzed cellulose effectively. The optimal ratio of extracellular BGL-to-cellulosome activity was determined to be in a range of 5.5 to 21.6. Despite the critical impact of BGL, both excessive BGL expression and its assembly on the cellulosome via type I cohesin-dockerin interaction led to reduced cellulosomal activity, which further confirmed the importance of coordinated BGL expression with the cellulosome. This study will further promote industrial CBS application in lignocellulose conversion. |
| 刊物名称： | BIORESOURCE TECHNOLOGY |
| 影响因子： | 6.669 |
| 全文链接： | https://www.sciencedirect.com/science/article/pii/S0960852421007811?via%3Dihub |
