论文
Coordinated beta-glucosidase activity with the cellulosome is effective for enhanced lignocellulose saccharification
第一作者: Qi, K (Qi, Kuan); Chen, C (Chen, Chao); Yan, F (Yan, Fei); Feng, YG (Feng, Yingang); Bayer, EA (Bayer, Edward A.); Kosugi, A (Kosugi, Akihiko); Cui, Q (Cui, Qiu); Liu, YJ (Liu, Ya-Jun);
联系作者: Qi, K (Qi, Kuan); Chen, C (Chen, Chao); Yan, F (Yan, Fei); Feng, YG (Feng, Yingang); Bayer, EA (Bayer, Edward A.); Kosugi, A (Kosugi, Akihiko); Cui, Q (Cui, Qiu); Liu, YJ (Liu, Ya-Jun);
发表年度: 2021
卷: 337
页: -
摘要: Consolidated bio-saccharification (CBS) technology employs cellulosome-producing bacterial cells, rather than fungal cellulases, as biocatalysts for cost-effective production of lignocellulosic sugars. Extracellular beta-glucosidase (BGL) expression in the whole-cell arsenal is indispensable, due to severe cellobiose inhibition of the cellulosome. However, high-level BGL expression in Clostridium thermocellum is challenging, and the optimal BGL production level for efficient cellulose saccharification is currently unknown. Herein, we obtained new CBS biocatalysts by transforming BGL-expressing plasmids into C. thermocellum, which produced abundant BGL proteins and hydrolyzed cellulose effectively. The optimal ratio of extracellular BGL-to-cellulosome activity was determined to be in a range of 5.5 to 21.6. Despite the critical impact of BGL, both excessive BGL expression and its assembly on the cellulosome via type I cohesin-dockerin interaction led to reduced cellulosomal activity, which further confirmed the importance of coordinated BGL expression with the cellulosome. This study will further promote industrial CBS application in lignocellulose conversion.
刊物名称: BIORESOURCE TECHNOLOGY
影响因子: 6.669
全文链接: https://www.sciencedirect.com/science/article/pii/S0960852421007811?via%3Dihub